Glucose Sensing by Time-Resolved Fluorescence of Sol-Gel Immobilized Glucose Oxidase
نویسندگان
چکیده
منابع مشابه
Glucose Sensing by Time-Resolved Fluorescence of Sol-Gel Immobilized Glucose Oxidase
A monolithic silica gel matrix with entrapped glucose oxidase (GOD) was constructed as a bioactive element in an optical biosensor for glucose determination. Intrinsic fluorescence of free and immobilised GOD was investigated in the visible range in presence of different glucose concentrations by time-resolved spectroscopy with time-correlated single-photon counting detector. A three-exponentia...
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Changes in steady-state UV fluorescence emission from free or immobilizedglucose oxidase have been investigated as a function of glucose concentration.Immobilized GOD has been obtained by entrapment into a gelatine membrane. Changes insteady-state UV fluorescence have been quantitatively characterized by means ofoptokinetic parameters and their values have been compared with those previouslyobt...
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ژورنال
عنوان ژورنال: Sensors
سال: 2011
ISSN: 1424-8220
DOI: 10.3390/s110403483